Trichoderma selective media composition(TSM)
Trichoderma, a widely occurring soil fungi known for its plant disease control capabilities, can be isolated and identified using specific media and methodologies. Trichoderma Selective Medium (TSM) is a specialized medium designed for the quantitative isolation of Trichoderma spp. from soil samples. It contains a low glucose level to promote rapid growth and sporulation of the fungi, along with compounds like pentachloronitrobenzene (PCNB) and rose bengal, which Trichoderma colonies can tolerate, enabling them to outcompete other fungi.
Trichoderma selective medium (TSM)
- Selection of TSM enables comparison between aggressive and non-aggressive
- Trichoderma groups. The antimicrobials chloramphenicol, streptomycin, quintozene, and propamocarb are added to the medium to highly select T. harzianum in compact colonies without visible contamination
The comprehension of the Trichoderma selective media composition (TSM) is imperative for our operational efficacy.
1. 0.2 g of MgSO4∙7H2O.
2. 0.9 g of K2HPO4.
3. 1.0 g of NH4NO3.
4. 0.15 g of KCl.
5. 0.15 g of rose bengal.
6. 3 g of glucose.
7. 20 g of agar.
8. 950 mL of distilled water
How can Trichoderma be isolated from soil?
One common method for isolating Trichoderma is the serial dilution technique, which involves diluting the soil sample in a sterile solution and spreading it on the selective media to facilitate the growth of Trichoderma colonies. Another method entails collecting soil samples from a depth of 15–20 cm, placing them in sterile containers, and processing them in the laboratory at 4 °C to maintain the viability of the Trichoderma spores for isolation.
For identification, conventional methods utilizing morphological and cultural characteristics are often employed. This may include examining the arrangement of conidiophores, phialides, and conidia, as well as observing colony color and growth patterns. Additionally, molecular techniques such as PCR and sequencing of specific genes like the internal transcribed spacer (ITS) region can be utilized for more precise identification.
These methods and media play a crucial role in effectively isolating and identifying Trichoderma species, offering valuable applications in agriculture, environmental management, and industry.